EKSPRESI RNA ORGAN MANDIBULAR KEPITING BAKAU Scylla olivacea

ABSTRAK
 

Farnesoate Acid Methyl Transferase (FAMeT) merupakan enzim yang berperan dalam mengubah AF menjadi MF, sehingga berperan dalam stimulasi reproduksi dan pergantian karapaks krustasea. Kajian ini dilakukan pada kepiting bakau jenis S. olivacea yang berada pada fase intermolt dan premolt. Dalam kajian ini proses ganti karapaks dapat diamati melalui ekspresi RNA penyandi FAMeT dalam OM. Ekspresi RNA penyandi FAMeT memperlihatkan perbedaan tiap fase yang diamati. Konsentrasi RNA hasil ekstraksi terendah pada sampel intermolt dan tertinggi saat premolt. Hasil visualisasi RNA penyandi FAMeT memperlihatkan amplikon RNA sepanjang 450 bp. Demikian juga, pengukuran konsentrasi RNA penyandi FAMeT menunjukkan peningkatan dari fase intermolt menuju premolt. 

Kata kunci: Ekspresi RNA, FAMeT, Intermolt, Organ Mandibular, Premolt

referensi:

Sunarti Y, Soejoedono RD, Mayasari NLPI, Tahya AM. 2016. RNA expression of farnesoic acid O-methyl transferase in mandibular organ of intermolt and premolt mud crabs Scylla olivacea. AACL Bioflux. 9(2):270-275.

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Tahya AM, Zairin MJr, Boediono A, Artika IM, Suprayudi MA. 2016. Expression of RNA encode
FAMeT in mandibular organ of mud crabs Scylla olivacea. IJ Pharmtech Resear. 9(3):219-223.

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RUMPUT LAUT SEBAGAI SUMBER KARBOHIDRAT DALAM PAKAN IKAN BANDENG

Seaweed as a source of carbohydrates in the feed of milkfish (Chanos chanos forsskal)

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Siti Aslamyah^1*, Muhammad Yusri Karim¹,  Badraeni¹, Akbar Marzuki Tahya^1,2.

Siti Aslamyah <siti_aslamyah_uh@yahoo.co.id >

¹Department of Aquaculture, Hasanuddin University, South Sulawesi, Indonesia

²Department of Aquaculture, Bogor Agricultural University, West Java, Indonesia.

Abstract

Seaweed is known to have many benefits as a source of nutrients in foods including in fish feed. Seaweed is known to influence the improvement of growth performance, nutrient absorption, the chemical composition of the body, fat metabolism and disease resistance. This study aimed to test several types of seaweed fermentation of the green and brown strains of Kappaphycus alvarezii, Gracillaria gigas, and Sargasum sp. as carbohydrates and its role in the growth, survival, digestibility of feed and chemical composition of the body of the fish juvenile.

The results showed an increase in growth, survival, level of digestibility and chemical composition of the body. The highest growth rates shown by seaweed Sargasum sp. amounting to 150.45 ± 10.06 provisional highest survival rate seen in the use of green strain K. alvarezii 66.67 ± 6.67, the level of digestibility of carbohydrates in the range of 50.22% - 57.19% and protein between 64.88% - 66.65%. The highest increase in glycogen in the liver looks at the use of green strain as much as 6.29 ± 0.89, while the muscles seen in the G. gigas group as much as 7.29 ± 2.05. These results indicate that the use of seaweed to the diet had a positive influence on fish juvenile.

Keywords: carbohydrates, growth, milkfish, seaweed, survival.

Reference: Aslamyah S, Karim MY, Badraeni, Tahya, AM. 2016. Seaweed as a source of carbohydrates in the feed of milkfish (Chanos chanos forsskal). IJ Pharmtech. 9(11): 64-67.